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PALM in 3D

Scientists led by HHMI researcher Harald Hess have built on the super-resolution technique, PALM, to create iPALM. Interferometric photoactivated localization microscopy allows for 3D images instead of the normal 2D with PALM. Light from the sample is captured from both above and below, and the two light beams are sent to a beam splitter that directs them to three different cameras. The amount of light is used to calculate the vertical position of each fluorescent molecule in the sample.

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