In today's PLoS ONE, researchers led by Andreas Sundquist describe a method called Short Read Assembly Protocol that they say will lead to inexpensive de novo sequencing of mammalian genomes. Using reads from next-gen sequencing technologies, this method uses a hierarchical sequencing strategy to produce a clone map and contigs so scientists can make more use of those short reads. The researchers, who include Mostafa Ronaghi and Pavel Pevzner, validated their protocol on portions of the human genome.
In Next-Gen Sequencing, Making Those Short Reads Useful
May 30, 2007