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Need to Clone? Try This!

Have a cloning project coming up and not sure which technique to use? Benchfly's Michael Gage recommends isothermal assembly reaction. While he was recently trying to attempt a five-point ligation, Gage says he decided to give the technique a shot. Basically, he says, the technique works by doing PCR using primers "such that each successive piece that you want to stitch together has about 20-30 bp of homology to the next piece." After the five-point ligation with isothermal assembly, about 50 percent of Gage's 100-200 colonies were correct. Isothermal assembly reaction is a "cloning hero," he says.

The Scan

Rise of BA.5

The New York Times reports that the Omicron subvariant BA.5 has become the dominant version of SARS-CoV-2 in the US.

UK Health Secretary Resigns

Sajid Javid, the UK health secretary, resigned along with Chancellor Rishi Sunak, saying they cannot work with Prime Minister Boris Johnson's government, CNN reports.

Clones From Freeze-Dried Cells

A team in Japan has cloned mice from freeze-dried skin cells, according to the Guardian.

Genome Research Papers on Craniosynostosis, Macaque Retrotransposition, More

In Genome Research this week: structural variants in craniosynostosis, LINE-1 activity in rhesus macaque brain, and more.