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Need to Clone? Try This!

Have a cloning project coming up and not sure which technique to use? Benchfly's Michael Gage recommends isothermal assembly reaction. While he was recently trying to attempt a five-point ligation, Gage says he decided to give the technique a shot. Basically, he says, the technique works by doing PCR using primers "such that each successive piece that you want to stitch together has about 20-30 bp of homology to the next piece." After the five-point ligation with isothermal assembly, about 50 percent of Gage's 100-200 colonies were correct. Isothermal assembly reaction is a "cloning hero," he says.

The Scan

Fertility Fraud Found

Consumer genetic testing has uncovered cases of fertility fraud that are leading to lawsuits, according to USA Today.

Ties Between Vigorous Exercise, ALS in Genetically At-Risk People

Regular strenuous exercise could contribute to motor neuron disease development among those already at genetic risk, Sky News reports.

Test Warning

The Guardian writes that the US regulators have warned against using a rapid COVID-19 test that is a key part of mass testing in the UK.

Science Papers Examine Feedback Mechanism Affecting Xist, Continuous Health Monitoring for Precision Medicine

In Science this week: analysis of cis confinement of the X-inactive specific transcript, and more.