Researchers in China used the CRISPR/Cas9 system to engineer monkeys with certain gene modifications.
As they reported in Cell this week, Nanjing Medical University's Jiahao Sha and colleagues injected both Cas9 mRNA and sgRNAs into one-cell stage cynomolgus monkey embryos. In particular, they were able to target two genes at once. Modified embryos were transferred into surrogate mothers.
Sequencing of 15 embryos indicated that eight of them had mutations in both targeted genes.