Bitesize Bio covers tips for cell-free protein synthesis, or in vitro translation. One, you get better protein yields; two, it's an optimal environment for protein production; and three, there's no concerns about cell viability. Nick also discusses the best expression systems for prokaryotic and eukaryotic cells. "The choice of the system should be determined not just by origin but also by the biological nature of the protein and the requirements of downstream applications," he writes.
Making In Vitro Translation Work
Mar 03, 2009