Studying gene function using RNAi can be difficult unless you can identify your novel hits from your off-target effects. On his blog, Keith Robison suggests pooling siRNAs so their on-target effects are additive and the off-target ones are reduced. To minimize siRNAs from affecting one another, he also suggests having the siRNAs compete for a limited target. He also points his readers to a paper in Nucleic Acid Research in which the researchers find that Ago2 protein levels affect siRNA competition, as well as that RISC levels vary between cell lines.
Make Your siRNAs Compete
Oct 03, 2007