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A Good Way to Save Money

Bitesize Bio takes you through a protocol to make your very own competent E. coli cells, which can be pretty expensive to buy. "One morning's work (with a bit of work ahead of time) is all it takes to make great electrocompetent E. coli prep," Nick writes, adding that you'd be able to get competencies of 1x1010, with a little practice. Some tips include: keeping everything fresh and chilled at all times, washing the cells in glycerol, starting with a lot of cells, and washing the glassware by hand before autoclaving to make sure that there is no detergent.

 

The Scan

Gone, But Now Reconstructed SARS-CoV-2 Genomes

In a preprint, a researcher describes his recovery of viral sequences that had been removed from a common database.

Rare Heart Inflammation Warning

The Food and Drug Administration is adding a warning about links between a rare inflammatory heart condition and two SARS-CoV-2 vaccines, Reuters reports.

Sandwich Sampling

The New York Times sent tuna sandwiches for PCR analysis.

Nature Papers Describe Gut Viruses, New Format for Storing Quantitative Genomic Data, More

In Nature this week: catalog of DNA viruses of the human gut microbiome, new dense depth data dump format to store quantitative genomic data, and more.