Do the Two-Step

Bitesize Bio covers the advantages that two-step RT-PCR reactions have over one-step qRT-PCR. These include reduced formation of primer dimers, reduced variability between two different RT reactions, less need to perform multiple reactions for housekeeping genes, increased flexibility in the amount of reverse transcriptase added to the reaction, and little intra-assay variation. In any case, the author recommends taking breaks for quality control purposes.

Get the full story

This story is free
for registered users

Registering provides access to this and other free content.

Register now.

Already have an account?
Login Now.

CNBC reports that Amazon invested in the startup Grail as it sees an opportunity for its cloud computing company in genomics.

Lawrence Krauss writes at Slate that science is needed for good public policy and should not be ignored.

Researchers are working on re-making the yeast genome from scratch, according to the Associated Press.

In Cell this week: functional profiling of Plasmodium genome, a self-inactivating rabies virus, and more.