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Do the Two-Step

Bitesize Bio covers the advantages that two-step RT-PCR reactions have over one-step qRT-PCR. These include reduced formation of primer dimers, reduced variability between two different RT reactions, less need to perform multiple reactions for housekeeping genes, increased flexibility in the amount of reverse transcriptase added to the reaction, and little intra-assay variation. In any case, the author recommends taking breaks for quality control purposes.

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UK Royal Statistical Society is organizing a working group to develop guidelines for assessing COVID-19 tests, the Guardian reports.

The Washington Post reports that the White House chief of staff has asked the US Food and Drug Administration to justify the stricter standards it is seeking for a coronavirus vaccine.

President Donald Trump's "good genes" comment raises eugenics concerns, CNN reports.

In PLOS this week: genetic analysis of tremor condition, analysis of a West and Central African tree used in traditional medicine, and more.