All the Network's Pieces

An article in this week's issue of Cell describes a new computational model, called NetworKIN, that can determine what kinase works at a phosphorylation site and that improves the accuracy with which signaling networks can be constructed. Researchers led by Rune Linding applied their approach of combining amino acid sequence analysis with text mining of signaling pathway and protein interaction databases to DNA damage signaling. They determined that 53BP1 is phosphorylated by CDK1 and Rad50 by ATM and predict that BCLAF1 is a GSK-3 substrate.