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Zyomyx, Affymetrix, Nanosphere, Sapporo Immuno Diagnostic Laboratory

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Zyomyx received US Patent No. 6,720,157, “Chips having elevated sample surfaces.” A chip is disclosed. The chip includes a base including a non-sample surface and at least one structure includes a pillar. Each structure includes a sample surface that is elevated with respect to the non-sample surface and each structure may be adapted to receive a sample.


Affymetrix received US Patent No. 6,720,149, “Methods for concurrently processing multiple biological chip assays.” The patent covers a method for processing multiple biological chip assays within test wells, with each well containing a biological chip with a molecular probe array; methods for introducing samples into the test wells; methods for subjecting the plate to manipulation by a fluid-handling device that automatically performs steps to carry out reactions between target molecules in the samples and probes; and subjecting the biological chip plate to a biological chip plate reader that interrogates the probe arrays to detect any reactions between target molecules and probes.


Nanosphere received US Patent No. 6,720,411 and 6,720,147, “Nanoparticles having oligonucleotides attached thereto and uses therefore.” The patents cover methods of detecting nucleic acids by contact with nanoscale particles with oligonucleotides attached. These oligos have sequences complementary to portions of the sequence of the nucleic acid. A detectable change, preferably a color change, is brought about as a result of hybridization. The invention also provides compositions and kits comprising particles. The invention further provides methods of synthesizing unique nanoparticle-oligonucleotide conjugates, the conjugates produced by the methods, and methods of using the conjugates. In addition, the invention provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from other nucleic acids.


Sapporo Immuno Diagnostic Laboratory received US Patent No. 6,720,164, “Method of determining substrate, and biosensor.” The patent covers a method of quantifying a substrate, by which the substrate contained in various samples can be conveniently and quickly quantified without resort to any pretreatments, and a biosensor in which the reaction reagent and the electrode system are integrated and which enables convenient and quick quantification. Additionally, the patent covers a method of quantifying a substrate in a sample by using an electrode system made of electrically conductive materials and a reaction reagent comprising at least a dehydrogenase, a coenzyme, an electron mediator, and a tetrazolium salt, which comprises performing an enzyme reaction and a redox reaction between the reaction reagent and the substrate in the sample, and detecting a formazan formed as the final reaction product.

 

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