Schott Nexterion of Duryea, Pa., last week introduced its Slide E epoxy slide product for covalent probe binding with microarrays. The product uses an active coating chemistry for the immobilization of oligonucleotides. The active layer, over a borosilicate glass, is made up of reactive epoxy groups that form a covalent bond with all the probe’s nucleophilic groups, without requiring UV cross-linking or reduction. An amino modification of the oligonucleotide is only necessary when spotting short oligonucleotides (less than or equal to 25 mers). Nexterion Slide E also enables the spotting of small spots and, consequently, high-density microarrays with regular and reproducible spot morphology. The surface chemistry is stable and remains active even during long spotting runs, the company said.
Jun 09, 2004