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Qiagen Joins the Grape Genome Bandwagon, Introduces 14K Grape Oligonucleotide Set


Qiagen last week announced the availability of the Array-Ready Oligo Set for grape, its first oligo product introduction for the year.

The product follows closely the announcement earlier this month that Affymetrix is also introducing a 20,000-probe grape (Vitis vinifera) microarray product, designed in collaboration with the International Grape Genome Consortia (see BAN 1/14/04).

The Qiagen product contains 14,562 probes that are 70-mers, and is based on sequence information from release 3 of the Grape Gene Index released Aug. 13 by the Institute of Genome Research.

The product will ship in 384-well plate formats with 600 picomol of probes per well. A control plate with 24 controls in 16 replicates is also included. Sample sets, which contain 96 optimized 70-mer oligos, are also available.

Each probe is melting-temperature normalized and includes a 5’ amino linker for substrate attachment.

The product rollout, said Nicole Brass-Heckel, Qiagen’s global product manager for microarrays, is a response to the availability of the sequencing information, and an effort to provide meaningful tools for a marketplace that is in transition.

“It is a time of change,” Brass-Heckel told BioArray News. “For years, most microarray users have only done gene-expression analysis with three or four organisms. Now people are interested in bacteria, or plant tissues, and other organisms.”

Qiagen offers oligo sets for 21 organisms, and subsets for poplar, and for human genes involved in cancer, signal transduction, apoptosis, stress and aging, and liver enzymes.

The company also has available a new pig genome oligo set, version 1, which contains 10,665 70-mer oligo probes for Sus scrofa. Other new oligo sets include Anthrax, Campylobacter jejuni, and Magnaporthe.


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