Agilent Technologies and Caliper Technologies have released the Protein 200 Plus Lab Chip kit, a protein that quantities and sizes proteins. The kit is designed for use with the Agilent 2100 Bioanalyzer, a unit that uses LabChips to analyze RNA, DNA, proteins, and cells. This kit, which costs $524, is an upgrade of the Protein 200 LabChip kit, and combines all reagents into a single kit. It also includes a pre-mixed sample buffer and reduces the reagent costs, the company said.
The kit is designed to quantitate proteins with molecular weights from 14 to 200 kDa, and to analyze ten protein samples in less than 30 minutes. The primary use, according to the companies, is to determine recombinant expression levels in cell lysates, for protein purification, and for antibody quality control. The software that goes with the Agilent 2100 bio-analyzer enables users to quantitate proteins absolutely, obtaining information about purity, quality, concentration, and size of proteins. It also allows users to define the calibration standards.
Amersham Biosciences has introduced the Lucidea Array Spotter, which includes pen matching, environmental controls, and slide positioning to ensure optimum spot quality and uniformity. The system is capable of depositing 150 spots across 75 slides for a single sample uptake of less than 200 nl, and can deliver over 172,000 spots per hour, the company said.
Gene Network Sciences of Ithaca, NY, has introduced its BioMine DNA microarray analysis software. The software includes a total of 14 algorithms, including the Bicluster algorithm, which is designed to link genes to conditions directly, as well as percolation and super-paramagnetic algorithms; and 11 others. Additionally, the software includes five statistical tools. The tools use non-parametric analysis methods, do not assume the data is distributed normally, and correct normalization functions for microarray data sets. The software also provides several visualization techniques, including 3-D PCA, projection pursuit, parallel coordinates and self-self plots; as well as an experiment design tool that determines the number of replicates needed to produce a particular confidence level in a data set.
BioMine is currently under evaluation at over 30 pharmaceutical and biotechnology companies, and academic institutions. GNS is offering a free trial of the software for download at its website, www.gnsbiotech.com. The trial allows users to run up to five test sessions.