Microarray-based resonance light scattering assay for detecting DNA methylation and human DNA methyltransferase simultaneously with high sensitivity.
Analyst. 2014 Jun 2. [Epub ahead of print]
Ma L, et al.
The authors developed a microarray-based resonance light scattering assay that relies on a combination of methylation-sensitive endonuclease and gold nanoparticle probes to sensitively distinguish DNA methylation levels and to detect human DNA methyltransferase 1.
Recombination locations and rates in beef cattle assessed from parent-offspring pairs.
Genet Sel Evol. 2014 May 29;46(1):34. [Epub ahead of print]
Weng Z, et al.
The authors used the Illumina BovineSNP50 BeadChip to investigate the influence of recombination on haplotype phasing, locate recombination hotspots, scan the genome for quantitative trait loci and identify candidate genes that influence recombination, and quantify the impact of recombination on the accuracy of genotype imputation in beef cattle.
A multiplex high-throughput gene expression assay to simultaneously detect disease and functional markers in induced pluripotent stem cell-derived retinal pigment epithelium.
Stem Cells Transl Med. 2014 May 29. [Epub ahead of print]
Ferrer M, et al.
The authors developed a multiplex high-throughput gene expression assay that simultaneously detects endogenous expression of multiple developmental, functional, and disease markers in iPS cell-derived retinal pigment epithelium and optimized protocols to differentiate iPS cell-derived RPE that was then grown in 96- and 384-well plates.