RNA-seq and expression microarray highlight different aspects of the fetal amniotic fluid transcriptome.
Prenat Diagn. 2014 May 23. [Epub ahead of print]
Zwemer L, et al.
The authors determined that Affymetrix expression microarrays provided a broader view of overall gene expression within the fetal amniotic fluid transcriptome, while Illumina HiSeq RNA sequencing revealed alternative splicing and specific pathways relevant to the developing fetus. The degraded nature of cell-free fetal RNA also presented technical challenges for the RNA-seq approach.
A duplex recombinant viral nucleoprotein microbead immunoassay for simultaneous detection of seroresponses to human respiratory syncytial virus and metapneumovirus infections.
J Virol Methods. 2014 May 22. [Epub ahead of print]
Zhang Y, et al.
In this study, human respiratory syncytial virus and human metapneumovirus nucleocapsid proteins were expressed in a baculovirus system and their performance compared with virus culture lysate antigen using paired serum specimens collected from symptomatic children. The proteins were also used to develop a duplex assay based on the Luminex bead array platform, where diagnostic rises in antibody levels could be determined simultaneously at a single serum dilution.
A single slide multiplex assay for the evaluation of classical Hodgkin lymphoma.
Am J Surg Pathol. 2014 May 22. [Epub ahead of print]
Hollman-Hewgley D, et al.
The authors assessed the capabilities of the hyperplexing MultiOmyx platform, which is used to analyze 60 antibodies on single tissue sections from formalin-fixed paraffin-embedded tissue, as an aid to the diagnosis of classical Hodgkin lymphoma. They determined the platform to be practical for routine diagnosis and argued that it may be particularly useful in cases in which the sample size is limited, few Hodgkin-like cells are present, or in CD30-positive lymphoma cases with difficult morphology.