Cross-platform comparison of glycan microarray formats.
Glycobiology. 2014 Apr 17. [Epub ahead of print]
Wang L, et al.
In this study, six different glycan microarray platforms with different modes of glycan presentation were compared using five well-known lectins. The authors developed a new universal threshold method to enable comparisons across the platforms. The strongest binders of each lectin were identified using the method across all platforms while identification of weaker binders was influenced by platform-specific factors, including presentation of determinants, array composition, and self-reported threshold methods.
Twist on protein microarrays: layering wax-patterned nitrocellulose to create customizable and separable arrays of multiplexed affinity columns.
Anal Chem. 2014 Apr 15. [Epub ahead of print]
de Lange V, et al.
The authors developed a new microarray platform to detect multiple proteins per sample. They combined forward- and reverse-phase microarrays into an innovative three-dimensional format, and the authors claim to have eliminated several limitations of traditional approaches, such as protein loss and cross-reactivity between detection antibodies.
Bimodal imprint chips for peptide screening: integration of high-throughput sequencing by MS and affinity analyses by surface plasmon resonance imaging.
Anal Chem. 2014 Apr 15;86(8):3703-7.
Wang W, et al.
The authors developed a bimodal imprint microarray system for whole-peptide screening. Using a silver-sputtered silicon chip fabricated with microwell arrays, they were able to trap and pattern the candidate peptide beads in a one-well-one-bead manner. Peptides on the beads were then photocleaved in situ and a portion of the peptide in each well was transferred to a gold-coated chip to print the peptide array for high-throughput affinity analyses by surface plasmon resonance imaging. The peptide left in the silver-sputtered chip was analyzed via in situ single bead sequencing by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.