Signature of backward replication slippage at the copy number variation junction.
J Hum Genet. 2014 Mar 20. [Epub ahead of print]
Ohye T, et al.
The authors used chromsomal microarrays to analyze the del(2)(q13q14.2) chromosomal junction site observed in a woman with a recurrent pregnancy loss. They were able to demarcate a 2.8 megabase deletion that does not appear in the database of human genomic variations.
Simultaneous detection of IgG antibodies associated with viral hemorrhagic fever by a multiplexed Luminex-based immunoassay.
Virus Res. 2014 Mar 12. [Epub ahead of print]
Wu W, et al.
The authors developed a multi-pathogen and high-throughput method that can simultaneously detect hemorrhagic fever virus-specific IgG antibodies using Luminex xMAP technology. Recombinant antigens of nine HFVs including Hantaan virus, Seoul virus, Puumala virus, Andes virus, Sin Nombre virus, Crimean-Congo hemorrhagic fever virus, Rift Valley fever virus, severe fever with thrombocytopenia syndrome bunyavirus, and dengue virus were produced and purified from a prokaryotic expression system and the influence of the coupling amount was investigated.
Transcriptome sequencing and profiling of expressed genes in cambial zone and differentiating xylem of Japanese cedar (Cryptomeria japonica).
BMC Genomics. 2014 Mar 20;15(1):219. [Epub ahead of print]
Mishima K, et al.
After identifying expressed sequence tags in Japanese cedar tissue undergoing xylogenesis, the authors designed a custom cDNA microarray to compare expression of highly regulated genes throughout a growing season. This led to identification of candidate genes involved both in wood formation and later cessation of growth and dormancy.