Characterization of fetal cells from the maternal circulation by microarray gene expression analysis – could the extravillous trophoblasts be a target for future cell-based non-invasive prenatal diagnosis?
Fetal Diagn Ther. 2013 Nov 9. [Epub ahead of print]
Hatt L, et al.
In this study, fetal cells were enriched from maternal blood by magnetic-activated cell sorting using the endothelial cell marker CD105 and identified by XY fluorescence in situ hybridization. Expression patterns were compared between fetal cells and maternal blood cells using stem cell microarray analysis. More than half of these are genes identified are known to be expressed in the placenta, especially in extravillous trophoblasts, or EVTs. The authors hypothesize that EVTs could be the basis for future cell-based non-invasive prenatal diagnosis.
Transcriptome sequencing and microarray design for functional genomics in the extremophile Arabidopsis relativeThellungiella salsuginea (Eutrema salsugineum).
BMC Genomics. 2013 Nov 14;14(1):793. [Epub ahead of print]
Lee Y, et al.
Thellungiella salsuginea has attracted interest as an alternative plant model species with high tolerance of various abiotic stresses, according to the authors. In this paper, they report the results of de novo transcriptome assembly and annotation based on pyrosequencing and development and validation of a T. salsuginea microarray. The developed microarrays were used to investigate transcriptional responses of T. Salsuginea and Arabidopsis during cold acclimation.
Microsphere-based immunoassay for the detection of azaspiracids.
Anal Biochem. 2013 Nov 8. [Epub ahead of print]
Rodríguez L, et al.
Azaspiracids, or AZAs, are a group of lipophilic toxins found in shellfish. The authors of this study developed a microsphere-based immunoassay for the detection of AZAs on the Luminex system. The method is based on the competition between AZA-2 immobilized onto the surface of microspheres and free AZAs for interaction with a monoclonal anti-azaspiracid antibody.