Targeted resequencing of HIV variants by microarray thermodynamics.
Nucleic Acids Res. 2013 Aug 8. [Epub ahead of print]
Hadiwikarta W, et al.
The authors used DNA microarrays and inputs from hybridization thermodynamics to resequence genomic subsets of HIV. Seven coded clinical samples were analyzed, and the microarray results were found to be concordant with Sanger sequencing data.
Surface engineering for long-term culturing of mesenchymal stem cell microarrays.
Biomacromolecules. 2013 Aug 12;14(8):2675-83.
Rasi Ghaemi S, et al.
The authors describe surface modifications to passivate cell microarrays, in order to adapt the platform to study stem cell behavior over long-term culture periods. The functionalization of glass slides with (3-glycidyloxypropyl) trimethoxysilane enabled covalent anchoring of extracellular matrix proteins on microscale spots printed by a robotic contact printer.
Automated regenerable microarray-based immunoassay for rapid parallel quantification of mycotoxins in cereals.
Anal Bioanal Chem. 2013 Aug;405(20):6405-15.
Oswald S, et al.
This paper describes the development of an automated flow-through multi-mycotoxin immunoassay based on the Munich Chip Reader 3 platform. Using the array, the authors were able to detect in parallel aflatoxins, ochratoxin A, deoxynivalenol, and fumonisins in cereal samples in a competitive indirect immunoassay format.