Analysis of methylation microarray for tissue specific detection.
Gene. 2014 Oct 1. pii: S0378-1119(14)01114-7.
Muangsub T, et al.
The authors collect 39 DNA methylation profiles generated using an Illumina HumanMethylation 27 BeadChip Kit containing 22 common tissues and involving 27,578 CpG loci across the human genome. They found 86 positions of tissue-specific methylation CpG that encompass 34 hypermethylated TSMs and 52 hypomethylated TSMs. They believe the loci could be useful for the selection of tissue-specific DNA markers as diagnostic tools.
Polymer nanopillar – gold arrays as surface enhanced Raman spectroscopy substrate for the simultaneous detection of multiple genes.
ACS Nano. 2014 Oct 3. [Epub ahead of print]
Picciolini S, et al.
In this study, two-dimensional nanopillar arrays with plasmonic crystal properties were optimized for surface enhanced raman spectroscopy and tested in a biochemical assay for the detection of multiple genetic leukemia biomarkers. Target nucleotides were detected in a microarray assay with labeled gold nanoparticles functionalized with different DNA and various Raman reporters.
Microarray-based detection of Salmonella Enteritidis genes involved in chicken reproductive tract colonization.
Appl Environ Microbiol. 2014 Oct 3. pii: AEM.02867-14. [Epub ahead of print]
Raspoet R, et al.
A microarray-based transposon library screen was used to identify genes that are essential for the persistence of Salmonella Enteritidis inside primary chicken oviduct gland cells in vitro and inside the reproductive tract in vivo. In total 81 genes, with a potential role in persistence in both the oviduct cells and the oviduct tissue, were identified.