Identification of genomic regions associated with feed efficiency in Nelore cattle.
BMC Genet. 2014 Sep 26;15(1):100. [Epub ahead of print]
de Oliveira P, et al.
The objective of this study was to identify genes and quantitative trait loci associated with components of feed efficiency in Nelore cattle using Illumina BovineHD BeadChip genotypes obtained from 593 Nelore steers. Among the positional candidate genes selected for feed efficiency identified were HRH4, ALDH7A1, APOA2, LIN7C, CXADR, ADAM12, and MAP7. A comparison with published results on Bos taurus cattle indicated that different QTLs and genes may be involved in the control of feed efficiency traits in this Nelore cattle population.
Ultrasensitive carbohydrate-peptide SPR imaging microarray for diagnosing IgE mediated peanut allergy.
Analyst. 2014 Sep 26. [Epub ahead of print]
Joshi A, et al.
The authors describe the creation of an immunoarray for immunoglobulin E antibodies in blood using both peptide and carbohydrate epitopes. The assay relies on a surface plasmon resonance imaging microarray with peptide and β-xylosyl glycoside epitopes from the major peanut allergen glycoprotein Arachis hypogaea h2. A monoclonal anti-IgE antibody was included as a positive control.
Site-specific, reversible and fluorescent immobilization of proteins on CrAsH-modified surfaces for microarray analytics.
Chem Commun (Camb). 2014 Sep 25;50(84):12761-4.
Schulte-Zweckel J, et al.
A novel technique for protein immobilization onto CrAsH-modified surfaces is presented. This approach enables an efficient, reversible, and fluorogenic immobilization of proteins. Moreover, expressed proteins can also be directly immobilized from cellular lysates without prior purification. The immobilized proteins are suitable for protein-protein interaction studies and the fluorescence enhancement upon immobilization can be employed for the direct detection of the immobilized protein without the need for secondary detection methods.