An ensemble-based approach to imputation of moderate-density genotypes for genomic selection with application to Angus cattle.
Genet Res (Camb). 2012 Jul 18:1-18.
Sun C, Wu XL, Weigel KA, et al.
The authors developed an approach to combine imputation results from several independent software packages, with each package serving as a basic classifier in an ensemble-based system. The approach was used on data from 3,078 registered Angus cattle, each genotyped with the Illumina BovineSNP50 BeadChip.
CYP2C9 and VKORC1 polymorphisms are differently distributed in the Brazilian population according to self-declared ethnicity or genetic ancestry.
Genet Test Mol Biomarkers. 2012 Jul 18. [Epub ahead of print]
Soares RA, Santos PC, Machado-Coelho GLet al.
Using the Affymetrix SNP 6.0, the authors found that allelic frequency for the VKORC1 polymorphism was differently distributed according to self-declared ethnicity, and the frequency of intermediate plus poor metabolizers was higher in populations of European descent compared to other Brazilian populations.
Polymorphisms in the glial glutamate transporter SLC1A2 are associated with essential tremor.
Neurology. 2012 Jul 17;79(3):243-8.
Thier S, Lorenz D, Nothnagel M, et al.
The authors used Affymetrix's SNP Array 6.0 to conducted a two-stage, genome-wide association study in a total of 990 subjects and 1,537 control subjects from Europe to identify genetic variants associated with essential tremor.
Microarray-guided discovery of two-photon (2P) small molecule probes for live-cell imaging of cysteinyl cathepsin activities.
Chem Commun (Camb). 2012 Jul 25;48(58):7304-6.
Na Z, Li L, Uttamchandani M, Yao SQ.
A microarray immobilized with 105 aldehyde-containing small molecules was screened against mammalian cell lysates over-expressing cathepsin L to identify two potent inhibitors, which were subsequently converted into cell-permeable probes capable of live-cell imaging of endogenous cysteinyl cathepsin activities by two-photon fluorescence microscopy.
Label-free microRNA profiling not biased by 3' end 2'-O-methylation.
Anal Chem. 2012 Jul 18. [Epub ahead of print]
Shen Y, Zheng KX, Duan D, Jiang L, Li J.
The authors used an internally developed, label- and enzyme-free assay, based on a stacking-hybridized universal tag microarray, to profile of both normal and methylated small RNA species.
MicroRNA profiling of primary high-grade soft tissue sarcomas.
Genes Chromosomes Cancer. 2012 Jul 18. [Epub ahead of print]
Renner M, Czwan E, Hartmann W, et al.
The authors performed a comprehensive miRNA expression analysis in a series of 76 untreated, primary high-grade soft tissue sarcomas representing eight subtypes, and in a panel of 15 representative sarcoma cell lines, using microarray technology.
On-chip synthesis of protein microarrays from DNA microarrays via coupled in vitro transcription and translation for surface plasmon resonance imaging biosensor applications.
J Am Chem Soc. 2012 Jul 17. [Epub ahead of print]
Seefeld TH, Halpern AR, Corn RM.
The authors describe the fabrication of protein microarrays from double-stranded DNA microarrays by a one-step, multiplexed enzymatic synthesis in an on-chip microfluidic format.