NuGen Technologies of San Carlos, Calif., has received US Patent No. 7,294,461, “Methods and compositions for transcription-based nucleic acid amplification.” The patent claims methods for isothermal exponential amplification of a target polynucleotide. The methods employ two transcription modules, the first module providing linear amplification resulting in RNA transcripts, and a second module providing for further amplification resulting in more RNA transcripts. In one aspect, the amplification of the first module is composite-primer based. In a second aspect, the amplification of the first module is based on target switching to generate a primer extension product with a promoter sequence. In all aspects, the RNA transcripts of the first transcription module are subjected to further amplification by creating an intermediate product comprising a double stranded promoter region from which transcription can occur. The patent also claims compositions and kits for practicing the described methods, as well as methods which use the amplification results.
Japan Software Management of Kanagawa, Japan, has received US Patent No. 7,294,462, “Method for detection of base sequence of interest.” The patent claims a method for the detection of a base sequence of interest in a sample DNA or RNA by: a) contacting sample DNA or RNA to probe DNAs or RNAs in an aqueous solution to form a hybridization complex; b) isolating the hybridization complex; c) dissociating the complex to recover the probe DNAs or RNAs; and d) identifying the probe DNAs or RNAs to detect a base sequence of interest in the sample DNA or RNA.
Rosetta Inpharmatics of Seattle has received US Patent No. 7,294,478, “Microarray reaction cartridge.” The patent claims an apparatus and method for the shipment, storage, and high-throughput processing of microarrays. According to the patent, the cartridge includes a body with a cavity defined by an outer surface and two dimple features in fluid communication with the cavity. The cavity includes at least one ledge for supporting a microarray of biological probes and a reaction chamber defined at least in part by the ledge. A plate covers the cavity and attaches to the outer surface of the body. Ports in the plate or the dimple features allow introduction of sample, reaction, and wash solutions into the reaction chamber so that the solutions contact the probes on the microarray. The disposable microarray cartridges are used to package and store microarrays prior to use and to process microarrays in a high-throughput manner. The footprint of the cartridge is designed to be compatible with standard robotic formats or standard re-formatting approaches, according to the patent.