Skip to main content
Premium Trial:

Request an Annual Quote

Nugen s IP Portfolio Details its Amplification Technology

Premium

Nugen’s amplification products are based on a set of related inventions by its co-founder, Nurith Kurn, who was formerly a research director at Dade-Behring Diagnostics. Nugen has so far published ten US patent applications and received one patent for these inventions. The patent and the applications describe in detail the company's single primer isothermal amplification, or SPIA, method, and related mechanisms for amplification of DNA.

The granted patent, US Patent No. 6,251,639, "Methods and compositions for linear isothermal amplification of polynucleotide sequences, using a RNA-DNA composite primer," decribes broadly the SPIA method and kits that use this method. This method involves a composite primer, primer extension, strand displacement, and an optional termination sequence to amplify cDNA, as well as a similar method for amplifying sense RNA using strand displacement, template switching, and a propromoter oligonucleotide, as well as a composite primer and primer extension.

The company has also applied for patents on methods for the production of partially double-stranded nucleic acids comprising a 3' overhang from an RNA target and use in methods of recombining polynucleotides (20030215926); additional methods for amplifying RNA (20030087251, 20020164628); methods for amplifying and detecting nucleic acid sequences generally (20030186234, 20030175785, 20020115088 20030017591, 20020058270), and specifically, methods for linear isothermal amplification of polynucleotide sequences (20010034048).

The Scan

Lung Cancer Response to Checkpoint Inhibitors Reflected in Circulating Tumor DNA

In non-small cell lung cancer patients, researchers find in JCO Precision Oncology that survival benefits after immune checkpoint blockade coincide with a dip in ctDNA levels.

Study Reviews Family, Provider Responses to Rapid Whole-Genome Sequencing Follow-up

Investigators identified in the European Journal of Human Genetics variable follow-up practices after rapid whole-genome sequencing.

BMI-Related Variants Show Age-Related Stability in UK Biobank Participants

Researchers followed body mass index variant stability with genomic structural equation modeling and genome-wide association studies of 40- to 72-year olds in PLOS Genetics.

Genome Sequences Reveal Range Mutations in Induced Pluripotent Stem Cells

Researchers in Nature Genetics detect somatic mutation variation across iPSCs generated from blood or skin fibroblast cell sources, along with selection for BCOR gene mutations.