A microarraying protocol developed by Niki Radulovich, a researcher at the University of Toronto using glass microarrays to spot RNA hybridizations on tumor tissues, is now being posted online. The protocol is designed for use of small volumes of RNA. Radulovich used 50 micrograms of RNA, and indirectly labeled the samples with Cy3 and Cy5 dyes. This indirect labeling procedure eliminated variations in incorporation rate between the two dyes. The protocol is now available in PDF format. The URL for direct download is http://zenith.berkeley.edu/genearrays/Files/NR_Microarray_Probe_Label.pdf.
New Protocol for Arraying Small Sample Volumes Available on Web
Jun 22, 2001