Plexpress said recently that it has expanded its library of prevalidated probes for multiplexed gene expression analysis on its Transcript Analysis with the aid of Affinity Capture, or TRAC, platform.
The update includes genes relevant for the investigation of in vitro drug interactions during preclinical studies, such as key members of the cytochrome P450 family as well as many membranous transport channels.
TRAC relies on a set of amplifiable detection probes and biotinylated oligo-capture probes that are hybridized in solution. The resulting sandwich hybrids are collected on magnetic streptavidin-coated microparticles and washed. The hybridized probes are then eluted and detected with laser-induced fluorescence and capillary electrophoresis. TRAC has been adapted to work in a 96-well format, allowing users to profile the expression of 30 genes in each well, according to Plexpress.