Molecular Devices, PamGene, Corning, Affymetrix, Agilent Technologies | GenomeWeb
Molecular Devices has received US Patent No. 7,364,846, “Gene expression profiling from FFPE samples.” The patent claims a method of synthesizing cDNA from the polyadenylated RNA from cells of a formalin-fixed and paraffin-embedded sample. The method includes a) extracting RNA from the cells of an FFPE sample; b) heating the RNA at about 70 degrees Celsius for 2 to 8 hours; and c) contacting the extracted RNA with a primer. The primer is linked to a promoter sequence and includes an oligo dT sequence. A cDNA strand is consequentially synthesized.

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