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Lucidea Array Spotter, Pan Oligo, ArrayPlate

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Amersham Pharmacia Biotech unveiled its new Lucidea Array Spotter at the Northwest Microarray Conference in Seattle last week. The spotter is designed to provide increased control of environmental conditions and the deposition of samples, the company said. The 24-pin spotter contains the arrays within a closed environment in order to enable the control of humidity.

The Lucidea Spotting Pens, made of stainless steel with a capillary design, are individually spring-loaded in order to speed up the deposition. The pins, which can uptake a maximum of 200 nanoliters and deposit a maximum of 100 picoliters, are also matched to one another in order to minimize variation between spots, the company said. Additionally, the arrayer uses a vacuum wash system that is designed to minimize carryover of sample.

 

Paradigm Genetics has developed a microarray for the rice pathogen Magnaporthe grisea. The chip, which was developed for Paradigm by Agilent Technologies, comprises a host of Magnaporthe gene sequences and positive and negative controls to help in data analysis, Paradigm said. Data on the microarray will be incorporated into Paradigm’s existing database of fungal organisms, which may be used to help discover and develop fungi.

 

MWG Biotech of Ebersberg, Germany, has introduced its Pan Oligo oligonucleotide sets for glass slide microarrays. The Pan Oligo sets are available for human, rat, yeast, and E. coli genomes and include the company’s buffer solutions and arraying protocol. A single set will produce anywhere from 1,000 to 4,000 microarrays, the company said. But sets can also be aliquotted to customers’ specifications. MWG additionally sells Pan Oligo test sets for 500 euro ($455).

 

High Throughput Genomics has released the ArrayPlate, a product that it designed to measure genes and proteins simultaneously using spatial arrays in microplates. The ArrayPlate involves putting multiple gene targets in each well of a 96- or 384-well microplate, along with nucleases, to enable researchers to conduct what it calls multiplex molecular profiling. The user images the entire plate to create a readout of the plate, and determinations are made using a readout of the datapoints. The company said the system could detect as few as 1,000 cells in 0.01 microgram of total RNA, and is reproducible at a three to 13 percent coefficient of variation.