Kenneth Loren Beattie of The Woodlands, Texas, has received US Patent Number 6,426,183, “Oligonucleotide microarrays: direct covalent attachment to glass.” The invention provides an improved method for stably attaching a compound, in particular one with at least one amine and hydroxyl group, to a silaceous or silane-containing substrate, in particular glass, porous silica, or oxidized silicon. The method is especially useful attaching nucleic acid sequences, e.g. oligonucleotide or PCR-generated DNA fragments.
The Regents of the University of Michigan in Ann Arbor have received US Patent Number 6,426,184, “Method and apparatus for chemical and biochemical reactions using photo-generated reagents.” The three inventors are co-founders of Xeotron of Houston, Texas. The patent covers a method for performing chemical or biochemical reactions in solution using in situ-generated photo-products as a reagent. This method has applications in the parallel synthesis of molecular sequence arrays on solid surfaces, such as oligonucleotides and peptides on microarrays. The invention also includes an apparatus for performing light-controlled reactions, where light patterns are generated using a computer and a digital optical projector.
Quark Biotech received US Patent Number 6,403,316, “Isolation of cDNA encoding for secreted or membranal proteins,” in June. The patent provides a method for identifying clones encoding for membrane and secreted proteins by deriving probes from membrane-bound polysomes and free polysomes and performing a microarray-based comparison of the relative abundance of the different RNA species. It also provides a method of augmenting a microarray analysis by utilizing RNA extracted from specific subcellular compartments as templates for DNA probes.