University College Dublin of Dublin, Ireland, has received US Patent No. 8,116,551, "Method and system for image analysis." A method for determining the level of expression of objects of interest using an automated image analysis system is claimed. The system is capable of assimilating the patterns of positively stained objects of interest, such as cells, and then using the detected patterns to segment the negative stained cells into relevant and irrelevant groups. The irrelevant group can then be eliminated from the analysis.