The National Research Council of Canada of Ottawa has received US Patent No. 8,057,852, "Microdevice for a fluorescence-based assay, and a method for making the microdevice." The method includes providing a substrate consisting of poly(cyclic olefin); protecting the substrate from ultraviolet light; and subjecting a surface of said UV-protected substrate to ozone oxidation to activate it; where intrinsic fluorophores on the surface of the substrate remain substantially unchanged after the ozone oxidation. The microdevice consist of a poly(cyclic olefin) body with an ozone-activated surface free of intrinsic fluorophores.
Gen9 of Cambridge, Mass., has received US Patent No. 8,058,004, "Microarray synthesis and assembly of gene-length polynucleotides." The patent claims a process for creating a mixture of oligonucleotide sequences in solution. The process includes synthesizing in situ or spotting oligonucleotide sequences on a microarray device or bead device, each having a solid or porous surface, where the sequences are attached to the surface. According to the patent, each sequence consists of a fragment of a target polynucleotide sequence as well as two flanking sequences, one at the 3' end and the other at the 5' end of each fragment. Each flanking sequence contains a primer region and a sequence segment having a restriction enzyme-cleavable site. Each oligonucleotide sequence is then amplified using primers complementary to the primer regions of the flanking sequence to form a number of double-stranded oligonucleotide sequences. The primer regions are then cleaved from the double-stranded oligonucleotide sequences at the restriction enzyme-cleavable sites to produce fragments of the target polynucleotide sequence, where the fragments together comprise the target polynucleotide sequence.
Honeywell International of Morristown, NJ, has received US Patent No. 8,058,005, "Method for single nucleotide polymorphism and mutation detection using real time polymerase chain reaction microarray." The method includes: a) hybridizing over a temperature gradient fluorescently tagged target amplicons to target nucleic acid probes immobilized in areas on an upper surface of a substrate, where the sequences of the probes differ so as to represent one or more SNPs; b) activating a fluorescence response from each of the fluorescently tagged target amplicons using an evanescent wave of a predetermined wavelength; c) detecting each fluorescence response; d) analyzing each fluorescence response; e) differentially hybridizing each of the amplicons to provide a melting curve; and f) analyzing each melting curve to qualitatively determine whether the target nucleic acid sequence has one or more SNPs.
Agilent Technologies of Santa Clara, Calif., has received US Patent No. 8,058,055, "High resolution chromosomal mapping." The patent describes methods of spatial and structural genomic analysis that can be used to map chromosomes and analyze the structure of chromosomal rearrangements, including the entire chromosome, as well as specific portions or regions of interest of the chromosomes. Multiple portions of the genome can be distinguished using a first detection entity and a second detection entity different from the first detection entity. The detection entities may be immobilized relative to oligonucleotides, which may be selected to bind to different locations within the chromosome. For instance, the oligonucleotides may be at least substantially complementary to the chromosome or substantially complementary to a specific location of the chromosome, according to the inventors.