Agilent Technologies has received US Patent No. 7,807,354, "Low volume hybridization." The patent claims a method of commingling a low volume of a target solution with an array. The method includes introducing a quantity of the target solution dissolved in an aqueous phase and a quantity of an immiscible liquid into a cavity that has an array on an interior surface. The method is carried out so that the quantities of target solution and immiscible liquid do not fill the volume and so that a gas is contained within the volume. The cavity is then agitated to form liquid bubbles of the immiscible liquid in the aqueous phase.
Agilent has also received US Patent No. 7,807,356, "Labeled nucleotide composition." The patent claims a method where a sample containing RNA is contacted with an enzyme that has an RNA ligation activity to provide labeled RNA. Methods of performing an array analysis of a labeled RNA sample are also described.
The University of Missouri of Columbia has received US Patent No. 7,807,358, "High-throughput methods for detecting DNA methylation." The patent claims a method called differential methylation hybridization that enables the high-throughput methylation analysis of multiple CpG island loci. The method relies on nucleic acid probes prepared from a cell sample to screen numerous CpG dinucleotide-rich fragments affixed on a screening array. Positive hybridization signals indicate the presence of methylated sites. Methods of preparing the hybridization probes and screening array are also provided.
The University of Michigan of Ann Arbor has received US Patent No. 7,807,807, "Linkers and co-coupling agents for optimization of oligonucleotide synthesis and purification on solid supports." The patent claims a method of modulation of synthesis capacity on and cleavage properties of synthetic oligomers from a solid support. The method uses linker molecules attached to a solid surface and co-coupling agents that have similar reactivities to the coupling compounds with the surface functional groups. The preferred linker molecules provide an increased density of polymers and more resistance to cleavage from the support surface, according to the patent, which claims that the method is useful for synthesis of oligonucleotides, oligonucleotide microarrays, peptides, and peptide microarrays.
Enzo Life Sciences of Farmingdale, NY, has received US Patent No. 7,807,352, "Process for producing two or more copies of nucleic acids in a library, and process for detecting or quantifiying more than one nucleic acid in a library." The patent claims processes for analyte detection, quantification, and amplification. Nucleic acid arrays and libraries of analytes are also claimed that make use of universal detection elements and signaling entities that are employed to detect and to quantify analytes. A means of amplifying target analytes is also provided.