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Life Technologies has received US Patent No. 7,727,716, "Detection of immobilized nucleic acid." The patent claims methods for determining the presence of immobilized nucleic acid using unsymmetrical cyanine dyes that are derivatives of thiazole orange, a staining solution, and select, non-genotoxic fluorogenic compounds. The methods include immobilizing nucleic acid on a support; contacting the nucleic acid with an unsymmetrical cyanine dye compound; and then illuminating the nucleic acid with an appropriate wavelength to detect its presence.

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