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Applied Biosystems, now Life Technologies, has received US Patent No. 7,575,863, "Methods, compositions, and kits comprising linker probes for quantifying polynucleotides." The patent claims methods, reagents, kits, and compositions for identifying and quantifying target polynucleotide sequences. A linker probe containing a 3' target specific portion, a loop, and a stem is first hybridized to a target polynucleotide and extended to form a reaction product that includes a reverse primer portion and the stem nucleotides.

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