Qiagen has received US Patent No. 7,553,619, "Detection method using dissociated rolling circle amplification." The patent claims methods for detecting small quantities of analytes such as proteins and peptides. The method involves associating a DNA circle with the analyte and the subsequent release and rolling circle replication of the circular DNA molecule. More specifically, an amplification target circle is associated with analytes using a conjugate of the circle and a specific binding molecule that is specific for the analyte to be detected. Amplification target circles not associated with the proteins are removed, and the the amplification target circles that are associated with the proteins are decoupled from the specific binding molecule and amplified by rolling circle amplification. The amplification is isothermic and can result in the production of a large amount of nucleic acid from each primer, according to the patent, serving as a detectable signal for the analytes.
The University of Michigan of Ann Arbor has received US Patent No. 7,553,958, "Linkers and co-coupling agents for optimization of oligonucleotide synthesis and purification on solid supports." The patent describes a method of modulation of synthesis capacity on a solid support, as well as the cleavage properties of synthetic oligomers from the solid support. The method uses linker molecules attached to a solid surface and co-coupling agents that are similarly reactive to the coupling compounds with the surface functional groups. The preferred linker molecules provide an increased density of polymers and are more resistance to cleavage from the support surface, the patent states. The inventors claim the method is useful for synthesis of oligonucleotides or peptide microarrays.
Enzo Life Sciences of Farmingdale, NY, has received US Patent No. 7,553,959, "Fluorescent dye composition and target labeled therewith." The patent describes labeling reagents, labeled targets, and processes for preparing labeling reagents. The labeling reagents can take the form of cyanine dyes, xanthene dyes, porphyrin dyes, coumarin dyes, or composite dyes, according to the patent. The labeling reagents are useful for labeling probes or targets, including nucleic acids and proteins and can be applied to protein and nucleic acid probe-based assays.
Stanford University has received US Patent No. 7,555,492, "System and method for internet-accessible tools and knowledge base for protocol design, metadata capture and laboratory experiment management." The patent claims a laboratory data management system, including a knowledge base that stores characteristics of laboratory reagents, experiment subjects, tissues and cells. An experiment input module that accepts input of parameters of an experiment and data from a knowledge base module is also claimed. Additionally, a protocol creator connected to the knowledge base module and the experiment input module is described, which creates a laboratory protocol for an experiment based on data supplied from the knowledge base module and the experiment input module.
BioMerieux has received US Patent No. 7,553,621, "Reading, detection or quantification method, hybrids or complexes used in said method and the biochip using same." The patent describes a method of reading, detecting, or quantifying a biological reaction on a support between a recognition molecule and a labeled target molecule or between a target molecule and a labeled detection molecule. The method includes treating the support under physicochemical conditions that allow the separation of the recognition molecule and the labeled target molecule, or the separation of the target molecule and the labeled detection molecule. Images produced before and after the physicochemical treatment can be used to determine the specific and non-specific bindings between the different molecules, the patent claims. The patent also describes hybrids and complexes used in the method and a biochip used to carry out the process.