Korea Electro Technology Research Institute of Changwon, Korea, has received US Patent No. 8,017,079, "Apparatus for and method of measuring bio-chips using uniform total internal reflection illumination." The described apparatus relies on a method for illuminating a biological sample through a side face of a substrate. A diffusion plate is used to form an evanescent field by illumination over the entire surface of the substrate so as to uniformly secure brightness of the illuminated light over a wide area of a substrate. The inventors claim the method is an efficient means of measuring fluorescence information from a biochip over a wide field of view.
Honeywell International of Morristown, NJ, has received US Patent No. 8,017,327, "Single nucleotide polymorphism genotyping detection via the real-time invader assay microarray platform." The method includes providing a linear or two-dimensional microarray of discrete regions, each having a defined area, formed on an upper surface of a solid support, where each region of the microarray includes a signal probe and an invader probe. Different samples containing an amplified target nucleic acid suspected of having a SNP at discrete regions in the microarray are also provided. In each region in the microarray the invader probe and the signal probe are annealed to the amplified target nucleic acid to provide a sample complex. The sample complex is then contacted with a flap endonuclease to activate a fluorescence response. According to the patent, fluorescence in a discrete region is indicative of the presence of the SNP in the sample.
Agilent Technologies of Santa Clara, Calif., has received US Patent No. 8,017,328, "Genome partitioning using a nicking endonuclease." The method includes nicking a region of the genome using a sequence-specific nicking endonuclease to produce a nicked double-stranded genomic region; hybridizing the nicked double-stranded genomic region with an oligonucleotide to produce a duplex in which a terminal nucleotide of the oligonucleotide lies adjacent to the nucleotide of the nick site; ligating the terminal nucleotide of the oligonucleotide to the nucleotide of the nick site to produce a ligation product; and separating the ligation product from unligated products using an affinity tag. According to the patent, the method may be employed to isolate a region of interest from genome for analysis by microarray, DNA sequencing, or PCR.
Panomics, now part of Affymetrix, of Santa Clara, Calif., has received US Patent No. 8,017,360, "Detection of nucleic acids through amplification of surrogate nucleic acids." Methods for detecting and optionally quantitating one or more target nucleic acids are provided. According to the patent, the surrogate nucleic acid is captured to each target nucleic acid, amplified, and detected. Kits and systems related to the methods are also described.
Narvalus Biotech of Torino, Italy, has received US Patent No. 8,017,367, "Biochip electroporator and its use in multi-site, single-cell electroporation." The patent describes an apparatus for the electroporation of any kind of cell adhering to a substrate at any stage of development, where an electrical signal can be driven and applied to a single adhering cell in culture in order to obtain its electroporation. The method to introduce genetic material or molecules of biological interest into a single adhering cell with the apparatus is also described.
NetBio of Waltham, Mass., has received US Patent No. 8,018,593, "Integrated nucleic acid analysis." A microfluidic system for performing nucleic acid analysis is claimed. The system can conduct sample collection, nucleic acid extraction and purification, amplification, sequencing, and separation and detection. The patent also claims optical detection systems and methods for separation and detection of biological molecules. These enable the simultaneous separation and detection of biological molecules, such as fluorescent dye-labeled nucleic acids, within microfluidic chambers or channels. The nucleic acids can be labeled with at least six dyes, each having a unique peak emission wavelength, according to the patent. The inventors claim that the systems and methods are useful for DNA fragment sizing applications such as human identification by genetic fingerprinting, as well as DNA sequencing applications.
Arborgen of Summerville, SC, has received US Patent No. 8,017,833, "Wood and cell wall gene microarray." The patent provides polynucleotide and polypeptide sequences isolated from Pinus radiata and Eucalyptus grandis that are involved in wood and cell wall biosynthesis. Methods for using the sequences, along with arrays, constructs, and transgenic plants, are also provided.
The University of Maryland of College Park, Md., has received US Patent No. 8,017,938, "Apparatus for microarray binding sensors having biological probe materials using carbon nanotube transistors." The claimed chip contains source and drain electrodes positioned on an array of carbon nanotube transistors that allow for electronic detection of nucleic acid hybridizations. Methods of oligonucleotide immobilization, electronically detecting biological materials using bound aptamers, and electronically determining relative abundances of specific target materials, are also claimed.