Conagra Grocery Products of Irvine, Calif., received US Patent 6,799,122, “Method for identifying polymorphic markers in population.” The patent covers a method for the identification of polymorphic markers in a population. The method includes genotypically characterizing a first sample of a population; selecting one or more individuals of the first sample based upon the genotypic characterization; fabricating a microarray with genomic DNA from each individual selected; and genotyping a second sample of the population using each fabricated microarray as a reference, thereby identifying the polymorphic markers in the population. Also provided is a method for the identification of polymorphic markers in a bacterial population. The method includes phenotypically characterizing a first sample of a population; selecting one or more individuals of the first sample based upon the phenotypic characterization; fabricating a microarray with genomic DNA from each individual selected, and genotyping a second sample of the population using each fabricated microarray as a reference, thereby identifying the polymorphic markers in the population.
Bioarray Solutions of Warren, N.J., received US Patent 6,797,524, “Light-controlled electrokinetic assembly of particles near surfaces.” The patent covers a method and apparatus for the manipulation of colloidal particulates and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled electrokinetic assembly of particles near surfaces relies on the combination of three functional elements: the AC electric field-induced assembly of planar aggregates; the patterning of the electrolyte/silicon oxide/silicon interface to exert spatial control over the assembly process; and the real-time control of the assembly process via external illumination. The present invention provides a set of fundamental operations enabling interactive control over the creation and placement of planar arrays of several types of particles and biomolecules and the manipulation of array shape and size. The present invention also enables sample preparation and handling for diagnostic assays and biochemical analysis in an array format, and the functional integration of these operations.
Yale University, based in New Haven, Conn., has received US Patent 6,797,474, “Rolling circle replication reporter systems.” Disclosed are compositions and a method for amplifying nucleic acid sequences useful for detecting the presence of molecules of interest. The method is useful for detecting specific nucleic acids in a sample with high specificity and sensitivity and has an inherently low level of background signal. A preferred form of the method consists of a DNA ligation operation, an amplification operation, and a detection operation. The DNA ligation operation circularizes a specially designed nucleic acid probe molecule. This operation is dependent on hybridization of the probe to a target sequence and forms circular probe molecules in proportion to the amount of target sequence present in a sample. The amplification operation is rolling circle replication of the circularized probe. A single round of amplification using rolling circle replication results in a large amplification of the circularized probe sequences. The amplified probe sequences are detected and quantified using any of the conventional detection systems for nucleic acids such as detection of fluorescent labels, enzyme-linked detection systems, antibody-mediated label detection, and detection of radioactive labels.
Eastman Kodak of Rochester, N.Y., receives US Patent 6,797,393, “Method for making biochip substrate.” The patent covers a gelatin-based substrate for fabricating protein arrays. The substrate containins: gelatin and a trifunctional compound A-L-B; wherein A is a functional group capable of interacting with the gelatin; L is a linking group capable of interacting with A and with B; and B is a functional group capable of interacting with a protein capture agent. A may be the same or different from B.