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Amersham Biosciences, Molecular Staging, Digene, NuGen Technologies, Solulink Bioscience, Clinical Micro Sensors


Amersham Biosciences received US Patent No. 6,686,161, “Methods and compositions for attachment of biomolecules to solid supports, hydrogels, and hydrogel arrays.” The patent covers solid supports (e.g., glass) and polymer hydrogels comprising reactive sites for the attachment of biomolecules, as well as biomolecules comprising one or more reactive sites for attachment to solid supports and polymer hydrogels. The invention provides novel compositions and methods for the preparation of biomolecules, solid supports, and polymer hydrogels comprising reactive sites.

Molecular Staging of New Haven, Conn., received US Patent No. 6,686,157, “Signal amplification with lollipop probes.” The patent covers a method for the detection of the amount and location of specific nucleic acid sequences, by use of a branched oligomer, referred to as a lollipop oligomer, which has three components that are joined at a common junction. The two arms at each end have sequences complementary to adjacent sequences in a target sequence. This allows the right and left arms to be ligated when the oligomer is hybridized to the target sequence, thus topologically linking the oligomer to the target sequence. The tail portion can then be detected at the location of the target sequence.

Digene of Gaithersburg, Md., received US Patent No. 6,686,151, “Immunological detection of RNA:DNA hybrids on microarrays.” The patent covers a hybridization method for the detection and measurement of biological molecules. A test sample containing the biological molecules of interest is hybridized with an unlabeled or, optionally, a detectably labeled complementary biomolecule to form a double-stranded hybrid immobilized to a solid phase. The immobilized hybrid may be detected with an entity which specifically recognizes an RNA:DNA hybrid, followed by analyses and quantification.

NuGen Technologies of Palo Alto, Calif., received US Patent No. 6,686,156, “Methods and compositions for transcription-based nucleic acid amplification.” The patent covers methods for isothermal exponential amplification of target polynucleotides. The methods employ two transcription modules, the first module providing linear amplification resulting in RNA transcripts, and a second module providing for further (generally cyclical) amplification resulting in more RNA transcripts. The RNA transcripts of the first transcription module are subjected to further amplification by creating an intermediate product comprising a double-stranded promoter region from which transcription can occur.

Solulink Bioscience of San Diego received US Patent No. 6,686,461, “Triphosphate oligonucleotide modification reagents and uses thereof.” The patent covers hydrazino, oxyamino, and carbonyl-based monomers and methods for incorporation them into oligonucleotides during enzymatic synthesis.

Clinical Micro Sensors of Pasadena, Calif., received US Patent No. 6,686,150, “Amplification of nucleic acids with electronic detection.” The invention describes compositions and methods for the detection of nucleic acids using a variety of amplification techniques, including both signal amplification and target amplification. Detection proceeds through the use of an electron transfer moiety that is associated with the nucleic acid to allow detection using an electrode.

The Scan

Genome Sequences Reveal Range Mutations in Induced Pluripotent Stem Cells

Researchers in Nature Genetics detect somatic mutation variation across iPSCs generated from blood or skin fibroblast cell sources, along with selection for BCOR gene mutations.

Researchers Reprogram Plant Roots With Synthetic Genetic Circuit Strategy

Root gene expression was altered with the help of genetic circuits built around a series of synthetic transcriptional regulators in the Nicotiana benthamiana plant in a Science paper.

Infectious Disease Tracking Study Compares Genome Sequencing Approaches

Researchers in BMC Genomics see advantages for capture-based Illumina sequencing and amplicon-based sequencing on the Nanopore instrument, depending on the situation or samples available.

LINE-1 Linked to Premature Aging Conditions

Researchers report in Science Translational Medicine that the accumulation of LINE-1 RNA contributes to premature aging conditions and that symptoms can be improved by targeting them.