Will off-target silencing prove too great a challenge to the future success of RNAi? | GenomeWeb

A.We have devised strategies to prioritize the potential genes of interest and, upon matching these criteria, we can examine the specificity of the siRNAs in our assays by re-ordering new sequences against additional regions of the same gene. When we have observed off-target effects, we can use bioinformatics approaches to determine the possible off-target gene that the siRNA species might be hitting and remake siRNAs specifically against this gene.

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