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Treasure Trove of Stem Cells

  • Title: Assistant Member, Fred Hutchinson Cancer Research Center; Assistant Professor, University of Washington
  • Education: MD, Harvard Medical School, 1996
  • Recommended by: Lee Hartwell

Seeing an entire lifetime of work ahead of yourself might be daunting for some people, but for Colleen Delaney, it’s the motivation that keeps her juggling several projects, both clinical and research-based. Delaney’s claim to fame — and one that will no doubt stay with her in coming years — is being an integral part of the first team that succeeded in rapidly growing cord blood stem cells, successfully transplanting them into a patient, and coaxing them to regenerate and repopulate the patient’s blood system.

Delaney, who began this work in 2002 as a member of Irwin Bernstein’s lab at the Fred Hutchinson Cancer Research Center, helped find a way to grow cord blood stem cells on a Notch ligand called Delta. The challenge with cord blood stem cells has long been that, while they’re an excellent source of cells for patients who need a bone marrow transplant, they grow slowly — too slowly to really have an impact on the transplant patient’s system. But Delaney’s procedure of growing the cells on the Delta protein increased the stem cell population 150-fold, a full two orders of magnitude beyond the state of art. “Being able to grow or culture stem cells in the lab is really the holy grail of stem cell biology,” says Delaney, who started out as a doctor but developed a passion for translational research. “We have the first demonstration that we can culture stem cells in a lab for clinical benefit.”

So Delaney wasted no time in taking her work straight to the clinic. After several months of work to appease the regulatory side, she launched a small clinical trial to see how these rapidly grown stem cells would fare in a patient. The first patient, like many who need bone marrow transplants, couldn’t find a match in the marrow donor system, and wound up in Delaney’s trial. “The first patient that we treated did unbelievably well — to the point that you’re slapping yourself and thinking, ‘This can’t be true,’” she says. “I don’t think I slept the entire 16 days we were culturing the first patient’s stem cells.”

Delaney says that the progress she has seen could ultimately be a boon to the more traditional ‘omics communities. “If we can provide the technology that allows us to [rapidly grow] a stem cell,” she says, “that then gives more cells for people who are doing proteomics or genomics or gene therapy.”

Looking ahead

Delaney recalls that when she first started in the field, her reason for doing so was simple: “I knew that there were going to be amazing discoveries in my lifetime,” she says, “and I wanted to be a part of that.” And here she is: now head of the cord blood transplant program she launched, Delaney says that next on the agenda is finishing up the pilot phase of her 15-patient stem cell clinical trial and moving on to phase two: “opening up this technology to other centers.” She also hopes to explore non-transplant uses for the technology. The pilot phase has another year and a half or so to go, Delaney says.

Publications of note

Delaney was first author on a paper in the journal Blood that described the early stages of the stem cell culturing work. Published in 2005, “Dose-dependent effects of the Notch Ligand Delta1 on marrow repopulating ability of cord blood cells” demonstrates the team’s effort to isolate donated cells and rapidly culture them in a lab setting.

And the Nobel goes to …

Winning a Nobel prize would be just as hard to believe as the rest of the ride Delaney has been on, so she figures she might as well win it for the stem cell self-renewal work she’s doing. “I’m so amazed that we’ve gotten as far as we have,” she says. “I’m right in the middle of the holy grail, and it’s still out there.”

The Scan

Genome Sequences Reveal Range Mutations in Induced Pluripotent Stem Cells

Researchers in Nature Genetics detect somatic mutation variation across iPSCs generated from blood or skin fibroblast cell sources, along with selection for BCOR gene mutations.

Researchers Reprogram Plant Roots With Synthetic Genetic Circuit Strategy

Root gene expression was altered with the help of genetic circuits built around a series of synthetic transcriptional regulators in the Nicotiana benthamiana plant in a Science paper.

Infectious Disease Tracking Study Compares Genome Sequencing Approaches

Researchers in BMC Genomics see advantages for capture-based Illumina sequencing and amplicon-based sequencing on the Nanopore instrument, depending on the situation or samples available.

LINE-1 Linked to Premature Aging Conditions

Researchers report in Science Translational Medicine that the accumulation of LINE-1 RNA contributes to premature aging conditions and that symptoms can be improved by targeting them.