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TIGR-Led Team Sequences Plant-Protecting Bacterium P. fluorescens

NEW YORK, June 27 (GenomeWeb News) - Researchers led by the Institute for Genomic Research have sequenced the genome of Pseudomonas fluorescens Pf-5, a root- and seed-dwelling bacterium that is believed to protect plants from diseases, according to the scientists.

According to the findings, which appear in the June 26 online issue of the journal Nature Biotechnology, the genome comprises one circular chromosome of 7,074,893 base pairs. The researchers, led by TIGR scientist Ian Paulsen, identified a total of 6,144 open reading frames.


The scientists also found that the genome is larger than those of the three other pseudomonads whose sequences have been published: P. aeruginosa PAO1, an "opportunistic animal pathogen;" P. putida KT2440, a saprophytic soil bacterium; and P. syringae DC300017, a plant pathogen.

"The genome sequence has helped us identify new chemical pathways that the microbe apparently uses to create what are known as 'secondary metabolites' -- possibly including new antibiotic compounds," Paulsen said in a statement.

Joyce Loper, a plant pathologist with USDA's Agricultural Research Service and the senior author of the Nature Biotechnology paper, said she believes the new data will help scientists develop new ways to "boost the effectiveness" of beneficial microbes against plant diseases.

"This genomic sequence reveals previously unknown traits of P. fluorescens that increase its potential for biological control," Loper said in the statement.

The P. fluorescens genome was sequenced at TIGR and analyzed by scientists there and at ARS and OregonStateUniversity. Researchers at Rutgers, WashingtonState University, and the Universityof Arizonacontributed to the study.

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