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TIGR Describes Gene-Enrichment Technique for Maize

NEW YORK, Dec. 19 (GenomeWeb News) - The Institute for Genome Research published a paper in today's issue of Science describing a method that enriches for gene-rich regions in the genome in order to aid in the sequencing of the maize genome, which has many highly-repetitive regions.

 

The method involves the use of two filtration techniques - methylation filtering and High-C0t selection - that together provide a four-fold reduction in the amount of sequencing necessary to find all of the maize genes, according to the researchers.

 

The High-C0t technique, developed by researchers at the University of Georgia's genetics department, excludes highly-repetitive DNA sequences by separating DNA segments into "low-copy" and "high-copy" portions.

 

The Scan

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