NEW YORK, Oct. 8 - A novel technique for generating expressed sequence tags promises to speed up both gene discovery and DNA sequencing, according to results of a recent study.

The technique relies on open-reading frame-expressed sequence tags, or ORESTES, which differ from conventional ESTs in that they provide a high proportion of sequence data from the central protein-coding region of a gene transcript rather than from its ends.

Also, ORESTES, which is based on a low-stringency RT-PCR approach, tend not to under represent genes with low expression levels.

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