Proteomics researchers are currently testing preliminary guidelines released by the gel electrophoresis module of the Proteomics Standards Initiative’s Minimum Information About a Proteomics Experiment standard, according to PSI officials.
Frank Gibson, the leader of MIAPE’s gel electrophoresis module, reported during the PSI’s spring workshop in San Francisco this April that the MIAPE-GE committee had finished gathering a requirements list, and had obtained comments about the list from a panel of experts on gel electrophoresis. Proteomics researchers are now testing the guidelines.
“The external reviewers said in general that the coverage [of the guidelines] is comprehensive and the clarity is excellent,” said Gibson. “Overall, they said, ‘Keep it at a minimum. Don’t add more.’”
Some of the MIAPE-GE reporting requirements include disclosing the date the experiment was performed; the person who performed the experiment; the name of the electrophoresis process used; the sample name; a description of the loading buffer; the number of dimensions of the electrophoresis process; the separation method employed for each dimension; a description of the gel matrix; the physical dimensions of the gel; a description of the protocol used to run the gel; a description of what was done between gel dimensions; a description of the protein/peptide detection process; the name of the image acquisition process; and a description of the gel image, including image name, dimensions, and resolution.
In the first stage of the MIAPE-GE testing, the requirements were simply listed in a Microsoft Word document, Gibson said. However, it would be a goal in later stages to have software programs that can automate the information-gathering process.
The gel electrophoresis module is one of nine MIAPE modules that have been formed so far. The PSI created the MIAPE guidelines in order to enable researchers to reproduce proteomics experiments originally performed by their peers, and to be able to reanalyze data from those experiments to answer different questions.
— Tien-Shun Lee
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