A. If the RNAi sequence is getting into the cell and just not knocking it down enough, we will try another sequence or a combination of sequences. Invitrogen sells “smart pools” of RNAi’s consisting of four or five sequences. Sometimes you can get better knockdown just by shifting the sequence a couple of nucleotides. If the RNAi isn’t getting into the cell (test by uptake of a labeled oligo, e.g. Block it by Invitrogen) we will try other transfection reagents or may engineer into a viral vector (e.g. lentivirus, herpesvirus, etc.).

Get the full story with
GenomeWeb Premium

Only $95 for the
first 90 days*

A trial upgrade to GenomeWeb Premium gives you full site access, interest-based email alerts, access to archives, and more. Never miss another important industry story.

Try GenomeWeb Premium now.

Already a GenomeWeb Premium member? Login Now.
Or, See if your institution qualifies for premium access.

*Before your trial expires, we’ll put together a custom quote with your long-term premium options.

Not ready for premium?

Register for Free Content
You can still register for access to our free content.

A new analysis examines the gender gap among paper authors in the sciences and says it may take decades or more to close.

Researchers have uncovered signals of selection that may enable the Bajau people to free five hundreds of feet deep, Reuters reports.

In Science this week: paternally inherited cis-regulatory structural variants in autism, and more.

A new report outlines issues facing the implementation of personalized medicine in the UK, the Independent reports.