Skip to main content
Premium Trial:

Request an Annual Quote

Proteomics Promises are Overplayed


There have been significant advances in genomics technology, but not enough to warrant the claims of some companies out to catalog human proteins. Or so say some proteomics forces, such as Ian Humphery-Smith, who argues that proteomics companies are promising more than they can deliver.

Humphery-Smith, a protein chemist at the University of Utrecht and the organizer of the Human Proteome Organization, spoke about these dilemmas at a June proteomics conference in Philadelphia. DNA microarrays will soon offer near-total coverage of the human genome on one chip, but current 2D gel and mass spectrometry technologies only allow researchers to catalog about five percent of the human proteome, he notes. “At present we’re at five percent and we’ve got 95 percent that are defying current technologies,” he says. “When [proteomics companies] have done 15 percent of 400,000 [proteins], or 15 percent of all the genes’ activity, then I’ll take back that criticism.”

“People have been overselling, putting their heads in the sand to steer away from the question,” he continues. “They’re more [than] happy to show you what they can do, but the task we have to do now is the human proteome, and we must scale up and think about getting the job done.”

Humphery-Smith says the key to getting at low-abundance proteins in cells is to design affinity agents, or molecules that capture certain classes of proteins, to screen out high-abundance proteins before attempting to analyze those that remain with mass spectrometry or other separation techniques.

“If you have antibodies or other ligands [to act as affinity agents], you have the potential to enrich your signal to do good mass spec,” he says. “But we can’t do PCR and we can’t hybridize [proteins] so we must have a ligand-affinity enrichment of signal.”

Ultimately, Humphery-Smith says, protein micro- array technology is the most likely to discover therapeutically important proteins because it will allow researchers to precisely study how the proteins in cells change with disease. “We’ve got to compete with Affymetrix and others,” he says, “until we’ve got a reporter matrix that can look at your proteome in six organs, before and after dinner, seven days of the week, with three drugs.”

— John S. MacNeil

The Scan

Study Points to Tuberculosis Protection by Gaucher Disease Mutation

A mutation linked to Gaucher disease in the Ashkenazi Jewish population appears to boost Mycobacterium tuberculosis resistance in a zebrafish model of the lysosomal storage condition, a new PNAS study finds.

SpliceVault Portal Provides Look at RNA Splicing Changes Linked to Genetic Variants

The portal, described in Nature Genetics, houses variant-related messenger RNA splicing insights drawn from RNA sequencing data in nearly 335,700 samples — a set known as the 300K-RNA resource.

Automated Sequencing Pipeline Appears to Allow Rapid SARS-CoV-2 Lineage Detection in Nevada Study

Researchers in the Journal of Molecular Diagnostics describe and assess a Clear Labs Dx automated workflow, sequencing, and bioinformatic analysis method for quickly identifying SARS-CoV-2 lineages.

UK Team Presents Genetic, Epigenetic Sequencing Method

Using enzymatic DNA preparation steps, researchers in Nature Biotechnology develop a strategy for sequencing DNA, along with 5-methylcytosine and 5-hydroxymethylcytosine, on existing sequencers.