This article has been changed and clarified to more accurately describe the Universal Linkage System.
NEW YORK, Aug. 3 (GenomeWeb News) - Kreatech has sub-licensed its Universal Linkage System to Schleicher & Schuell for incorporation into Schleicher & Schuell's two-color labeling and fluorescent detection kit for its serum biomarker chip, the companies said today.
The Universal Linkage System includes the ULS molecule, which consists of a platinum complex, a detectable molecule, and a leaving group that is displaced when the target reacts with the molecule, Kreatech said. This molecule bings to the N7 position of guanine to form a label for DNA and RNA, and in proteins, binds to nitrogen and sulphur that contains side chains of methionine, cysteine, and histidine. The ULS can then be coupled to labels such as fluorochromes, biotin, dinitrophenol, and horse radish peroxidase.
With the serum biomarker chip, each sample is labeled with either biotin or fluorescein, and then the samples are pooled and exposed to the antibody microarray so they can competitively bind to the 120 different antibodies spotted on the chip. Bound labeled proteins are detected using a streptavidin dye conjugate or the anti-fluorescein dye conjugate.
The ULS labeling system, according to the companies, minimizes background fluorescence because it uses indirect fluorescence detection.
The companies did not disclose the financial terms of the agreement.