NEW YORK, May 11 – The US Department of Energy’s Joint Genome Institute has changed its DNA template preparation procedure to a method using Amersham Pharmacia Biotech’s TempliPhi DNA amplification kit, the company said Friday.
The Joint Genome Institute, which beta-tested TempliPhi, began implementing the technology three weeks ago and plans to have its production process converted over and fully automated by the end of the month. The JGI is using the technology to improve productivity, according to a statement.
The TempliPhi technology uses Rolling Circle Amplification and a DNA polymerase licensed exclusively to Amersham to grow bacteria and purify the template before sequencing. When fully automated, the technology allows researchers to produce DNA in four hours and in two steps. The technique is seen as a potential replacement for PCR.
At the 14 th Genome Sequencing and Biology meeting in Cold Spring Harbor, New York, the JGI presented data showing that compared to their old method, a standard industry protocol, the new method increased the pass rate by more than 10 percent and the read rate by more than 20 percent.
The number of full-time equivalent employees required for isolating the template also dropped 60 percent with the new technology.