NEW YORK, Dec. 19 (GenomeWeb News) - Helicos BioSciences said today that it has sequenced the genome of the M13 bacteriophage with its non-amplified True Single Molecule Sequencing technology.
The tSMS technology was used in conjunction with Helicos' SimplePrep genome preparation technique.
Helicos said that tSMS, which enables direct interrogation of a single molecule without amplication, offers a number of benefits, including no PCR bias, fewer errors, and no dephasing issues. The company said that the method costs around 1,000 times less than Sanger sequencing.
Sequencing of M13 was conducted on the entire genome, including homopolymeric sequences. The M13 genome is 6,400 base pairs long and encodes for 10 genes.