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Genome Technology s Peer Review: A Weekly Forum of Recently Published Journal Articles: Mar 8, 2005

Following is a description of a recently published research paper recommended by a scientist involved in integrated biology. The recommender, Jeff Skolnick, is director of the BuffaloCenterfor Excellence in Bioinformatics at the The State University of New York at Buffalo.


For a complete list of recommended papers, read Genome Technology, a GenomeWeb News sister publication.

Regulation of gene expression by a metabolic enzyme. Hall D, Zhu H, Zhu X, Royce T, Gerstein M, Snyder M. Science 15 October 2004; 306: 482-484.


Using a yeast proteome microarray, Michael Snyder and his colleagues at YaleUniversityhave discovered that Arg5,6, a mitochondrial enzyme involved in arginine biosynthesis, has DNA binding activity. Chromatin immunoprecipitation experiments produced evidence that Arg5,6 is associated with specific nuclear and mitochondrial loci in vivo, and deletion of Arg5,6 causes altered transcript levels of both nuclear and mitochondrial target genes.


The work is significant because it is the first example of a metabolic enzyme directly regulating eukaryotic gene expression, says Jeff Skolnick, director of the BuffaloCenterfor Excellence in Bioinformatics at the University at Buffalo. Gene products with enzymatic and structural functions are known to regulate gene expression indirectly, but until now it wasn't clear how important a regulatory role they play. The research is also notable because it relies --- at least in part --- on the yeast proteome array, a relatively new tool developed in Snyder's lab for probing protein function.